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Title   생물학적 치료 목표로서 위암에서 Midkine 발현의 탐색 ( Evaluation of Biologic Phenotype by Midkine Gene Expression in Gastric Cancer as a Target for Biotherapy )
Publicationinfo   1997 Jan; 029(01): 69-81.
Key_word   Gastric cancer, Midkine, Pentosan polysulfate, Biotherapy
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Abstract   Purpose: We studied biological phenotypes of gastric cancer cell lines based on a novel heparin-binding growth/differentiation factor, midkine (MK) expression. Materials and Methods: Nine gastric cancer cell lines and 25 gastric cancer tissues were tested for MK expression by Northern blot analysis. Soft agar assay for in vitro tumorigenesis, cross-feeding assay for paracrine angiogenic activity, ELISA for uPA and PAI-1 measurement were performed. Results: MK expression was found in 67% (6/9) of the gastric cancer cell lines, and 56% (14/25) of the primary gastric cancer tissues. Gastric cancer cell lines with MK expression were more tumorigenic in soft agar assay and endothelial cell growth stimulatory in cross-feeding assay than cells which did not express MK. However, urokinase-type plasminogen activator (uPA) expression did not correlate with MK expression. Growth of MK expressing cells was inhibited by a heparin-binding blocking agent, pentosan polysulfate (PPS). In cancer tissues, MK expression correlated with tumor size, suggesting in vivo autocrine and paracrine activity. Conclusion: Gastric cancer cells with increased MK gene expression showed increased tumorigenic and angiogenic activity. Therefore, this proliferation promoting activity of MK can be targeted by an anti-heparin binding agent as a biotherapy model in gastric cancer treatment.
저 자   정현철(Hyun Cheol Chung),라선영(Sun Young Rha),정희철(Hei Cheol Chung),곽현주(Hyun Joo Kwak),조재웅(Jae Young Cho),공수정(Soo Jung Gong),노성훈(Sung Hoon Noh),김주항(Joo Hang Kim),노재경(Jae Kyung Roh),민진식(Jin Sik Min),김병수(Byung Soo Kim)